Coding
Part:BBa_K1975005:Design
Designed by: Joachim Steen Larsen Group: iGEM16_UNIK_Copenhagen (2016-10-05)
Cyan Fluorescent Protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 694
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To secure the optimal expression we choose to make a codon optimization of the genes. This was done through the Codon Optimization tool at IDT's webpage.
Source
The sequence for CFP genomic DNA from Acropora aculeus. This sequence has also been optimized for expression in B. subtilis.